Date & Time: Thu.16 May., 2019 16:20-17:50
Place: Engineering 108 Lecture Room, Higashi-Hiroshima Campus, Hiroshima University
Commentary: Yukihiko MATSUMURA
Professor, Graduate School of Engineering, Hiroshima University
M1 Graduate School of Engineering, Hiroshima University
“Detection of Formic Acid from Hydrothermal Reaction Field by Using In-Situ Mass Spectrometry”
Hydrothermal reaction field is often employed for biomass conversion, but due to its high temperature and pressure, it is not easy to detect the chemical species in the reactor. Products are often analyzed after cooling down and depressurization, but the possibility of reaction during these steps cannot be denied. Thus, in-situ mass spectrometry has been proposed to directly analyze the chemical species in the reactor. However, so far this technology can be applied only to temperature lower than 250 ℃. In this study, we improved the operation so that analysis from the reactor temperature or 400 ℃ is successfully conducted. Decomposition of formic acid was observed.
Lecture: Mojarrad Mohammad
D2 Graduate School of Integrated Sciences for Life, Hiroshima University
“Simultaneous production of 3-Hydroxypropionic acid and 1,3-Propanediol by psychrophile-based simple biocatalysts in Shewanella sp.”
This study examines the potential of the Psychrophile-based Simple bioCatalyst approach in the production of 3-HP and 1,3-PDO compounds. The transformed psychrophile host bacteria by DhaB,DhaT, and PuuC, were tested and the desired result to measure the enzymatic activity for aldehyde dehydrogenase in transformed bacteria was obtained when the concentration of NADH was 0.5 and 1 mM, the optimum temperature was 40° C. All samples should be placed at 45° C for 15 minutes before measuring enzymatic activity. The same results revealed for both transformed bacteria by DhaT. We also are going to examine the potential of the PSCat approach in the production of and the co-production of these compounds.
Lecture: Takahisa TAJIMA
Assistant Professor, Graduate School of Integrated Sciences for Life, Hiroshima University
“Itaconic acid production by Psychrophile-based Simple biocatalyst”
We constructed “psychrophile-base simple biocatalyst (PSCat)” to produce valuable chemicals effectively, because heat treatment of the cells have positive effects on the production; inactivation of the host metabolic enzymes producing byproducts and improvement of the cell membrane permeability of substrates. High yield itaconic acid production was achieved by the PSCat heterologously expressing two mesophilic conversion enzymes in the psychrophilic Shewanella sp. We are trying to improve the conversion process by immobilization of PSCat and creating fusion proteins of conversion enzymes.